A Homologous Radioimmunoassay for Chicken Insulin
نویسندگان
چکیده
منابع مشابه
Assessment of developmental changes in chicken and turkey insulin-like growth factor-II by homologous radioimmunoassay.
The development of a homologous RIA for chicken insulin-like growth factor-II (cIGF-II) and its application to investigate the developmental changes in IGF-II in the chicken and turkey are described. A double-antibody RIA has been developed using recombinantly derived cIGF-II as antigen, radiolabelled tracer and standard. Serial dilutions of chicken and turkey plasma were parallel to serial dil...
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A double antibody radioimmunoassay (RIA) for the measurement of bullfrog basic gonadotropin (GTH) is described. The antiserum was raised against a highly purified bullfrog basic GTH. Bullfrog basic GTH-IV (pI=9.33) was used for radioiodination and as a reference standard. The dissociation constant of the binding of the antibody to bullfrog basic GTH-IV was 8.28×10-12M. The standard curve was ob...
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Osteocalcin or bone gamma-glutamic acid-containing protein (GLA protein) was isolated from human bone and used to develop a homologous radioimmunoassay of human osteocalcin. The effect of age on serum osteocalcin was studied in 380 normal children and adolescents and 330 normal adults. The mean (+/- SD) values in adults were higher in men [25 +/- 5 micrograms/L (4.3 +/- 0.8 nmol/L)] than in pre...
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This article describes the synthesis of a pure cortisol-3-(O-carboxymethyl) oxime with its subsequent use in producing highly specific antibodies and a 125I-tracer with a shelf life in excess of several weeks. An assay was developed using a pH of 4, thus allowing a direct measurement of cortisol in serum without extraction or heat denaturation. The quality-control parameters were checked - the ...
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In this sensitive, specific 125I radioimmunoassay for plasma progesterone, an identical glucuronide bridge is used in the immunogen and radioligand. This apparently provides a system in which the bridge is poorly recognized and thereby avoids the excessively high affinity of the tracer for the antiserum and the consequent poor sensitivity that commonly is found in other homologous-bridge situat...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 1983
ISSN: 0032-5791
DOI: 10.3382/ps.0620697